특허 및 논문

Check out the archives at Biosolvix.
bt_bb_section_bottom_section_coverage_image

BIOSOLVIX ARCHIVE특허 및 논문

Ultraefficient extracellular vesicle–guided direct reprogramming of fibroblasts into functional cardiomyocytes.

Author
관리자
Date
2024-02-02 08:37
Views
359
Hyosuk Kim, Byeong-Wook Song, Soon-Jung Park, Seong Woo Choi, Hanbyeol Moon, Ki-Chul Hwang, Sun-Woong Kang, Sung-Hwan Moon, Yoosoo Yang,Ick Chan Kwon,,Sun Hwa Kim. Ultraefficient extracellular vesicle–guided direct reprogramming of fibroblasts into functional cardiomyocytes. SCIENCE Adv.2022. 8, eabj6621.

 

Abstract



Direct lineage conversion holds great promise in the regenerative medicine field for restoring damaged tissues using functionally engineered counterparts. However, current methods of direct lineage conversion, even those using virus-mediated transgenic expression of tumorigenic factors, are extremely inefficient (~25%). Thus, advanced methodologies capable of revolutionizing efficiency and addressing safety concerns are key to clinical translation of these technologies. Here, we propose an extracellular vesicle (EV)-guided, nonviral, direct lineage conversion strategy to enhance transdifferentiation of fibroblasts to induced cardiomyocyte-like cells (iCMs). The resulting iCMs have typical cardiac Ca2+ transients and electrophysiological features and exhibit global gene expression profiles similar to those of cardiomyocytes. This is the first demonstration of the use of EVs derived from embryonic stem cells undergoing cardiac differentiation as biomimetic tools to induce cardiac reprogramming with extremely high efficiency (>60%), establishing a general, more readily accessible platform for generating a variety of specialized somatic cells through direct lineage conversion


 

논문확인(클릭시 이동) 

 
Total 52
Number Title Author Date Votes Views
52
[특허] 콘케이브와 진탕배양 기법으로 형성된 전분화능 줄기세포 유래 신경세포 제조방법 및 이에 의해 제조된 3D 신경 오가노이드를 통한 약물 독성 평가방법
관리자 | 2024.02.22 | Votes 0 | Views 257
관리자 2024.02.22 0 257
51
[특허] 중간엽줄기세포 패치 공배양 기법을 이용한 투명 배상체 유래 간세포 제조방법 및 이에 의해 제조된 3D 간 오가노이드를 통한 약물 독성 평가방법
관리자 | 2024.02.22 | Votes 0 | Views 279
관리자 2024.02.22 0 279
50
[특허] 인간 전분화능 줄기세포로 유래된 심장 오가노이드 제조방법, 효능 및 독성 평가 기술
관리자 | 2024.02.22 | Votes 0 | Views 311
관리자 2024.02.22 0 311
49
[특허] 인공 신경망을 이용한 심장 부정맥 유발약물의 스크리닝 방법
관리자 | 2024.02.20 | Votes 0 | Views 317
관리자 2024.02.20 0 317
48
[특허] 3 차원 세포 배양용 미세유체칩 및 이를 이용한 3 차원 세포 배양방법
관리자 | 2024.02.20 | Votes 0 | Views 287
관리자 2024.02.20 0 287
47
Translation reinitiating in c.453delC frameshift mutation of KCNH2 producing functional hERG K+ channels with mild dominant neg
관리자 | 2024.02.02 | Votes 0 | Views 285
관리자 2024.02.02 0 285
46
Extracellular matrix cues regulate the differentiation of pluripotent stem cell-derived endothelial cells.
관리자 | 2024.02.02 | Votes 0 | Views 341
관리자 2024.02.02 0 341
45
Ultraefficient extracellular vesicle–guided direct reprogramming of fibroblasts into functional cardiomyocytes.
관리자 | 2024.02.02 | Votes 0 | Views 359
관리자 2024.02.02 0 359
44
In vitro maturation of human pluripotent stem cell-derived cardiomyocyte: A promising approach for cell therapy.
관리자 | 2024.02.02 | Votes 0 | Views 372
관리자 2024.02.02 0 372
43
Enhancement strategy for effective vascular regeneration following myocardial infarction through a dual stem cell approach.
관리자 | 2024.02.02 | Votes 0 | Views 316
관리자 2024.02.02 0 316