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Enhancement strategy for effective vascular regeneration following myocardial infarction through a dual stem cell approach.

Author
관리자
Date
2024-02-02 08:29
Views
313
Hyeok Kim*, Soon-Jung Park*, Jae-Hyun Park, Sunghun Lee, Bong-Woo Park, Soon Min Lee, Ji-Won Hwang, Jin-Ju Kim, Byeongmin Kang, Woo-Sup Sim, Hyo-Jin Kim, Seung Hwan Jeon, Dong-Bin Kim, Jinah Jang, Dong-Woo Cho, Sung-Hwan Moon*, Hun-Jun Park*, Kiwon Ban*. Enhancement strategy for effective vascular regeneration following myocardial infarction through a dual stem cell approach. Experimental & Molecular Medicine volume 54, pages1165–1178 (2022).

 

Abstract



Since an impaired coronary blood supply following myocardial infarction (MI) negatively affects heart function, therapeutic neovascularization is considered one of the major therapeutic strategies for cell-based cardiac repair. Here, to more effectively achieve therapeutic neovascularization in ischemic hearts, we developed a dual stem cell approach for effective vascular regeneration by utilizing two distinct types of stem cells, CD31+-endothelial cells derived from human induced pluripotent stem cells (hiPSC-ECs) and engineered human mesenchymal stem cells that continuously secrete stromal derived factor-1α (SDF-eMSCs), to simultaneously promote natal vasculogenesis and angiogenesis, two core mechanisms of neovascularization. To induce more comprehensive vascular regeneration, we intramyocardially injected hiPSC-ECs to produce de novo vessels, possibly via vasculogenesis, and a 3D cardiac patch encapsulating SDF-eMSCs (SDF-eMSC-PA) to enhance angiogenesis through prolonged secretion of paracrine factors, including SDF-1α, was implanted into the epicardium of ischemic hearts. We verified that hiPSC-ECs directly contribute to de novo vessel formation in ischemic hearts, resulting in enhanced cardiac function. In addition, the concomitant implantation of SDF1α-eMSC-PAs substantially improved the survival, retention, and vasculogenic potential of hiPSC-ECs, ultimately achieving more comprehensive neovascularization in the MI hearts. Of note, the newly formed vessels through the dual stem cell approach were significantly larger and more functional than those formed by hiPSC-ECs alone. In conclusion, these results provide compelling evidence that our strategy for effective vascular regeneration can be an effective means to treat ischemic heart disease.


 

논문확인(클릭시 이동) 

 
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Enhancement strategy for effective vascular regeneration following myocardial infarction through a dual stem cell approach.
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