특허 및 논문

바이오솔빅스에 자료실을 확인해 보세요.
bt_bb_section_bottom_section_coverage_image

BIOSOLVIX ARCHIVE특허 및 논문

Predicting in vivo therapeutic efficacy of bioorthogonally labeled endothelial progenitor cells in hind limb ischemia models vi

작성자
관리자
작성일
2024-02-01 22:01
조회
62

Lim SH, Yoon HY, Park SJ, Song S, Shim MK, Yang S, Kang SW, Lim DK, Kim BS, Moon SH*, Kim KM*. Predicting in vivo therapeutic efficacy of bioorthogonally labeled endothelial progenitor cells in hind limb ischemia models via non-invasive fluorescence molecular tomography. Biomaterials.2021 Jan;266:120472.


Abstract


Human embryonic stem cells-derived endothelial progenitor cells (hEPCs) were utilized as cell therapeutics for the treatment of ischemic diseases. However, in vivo tracking of hEPCs for predicting their therapeutic efficacy is very difficult. Herein, we developed bioorthogonal labeling strategy of hEPCs that could non-invasively track them after transplantation in hind limb ischemia models. First, hEPCs were treated with tetraacylated N-azidomannosamine (Ac4ManNAz) for generating unnatural azide groups on the hEPCs surface. Second, near-infrared fluorescence (NIRF) dye, Cy5, conjugated dibenzocylooctyne (DBCO-Cy5) was chemically conjugated to the azide groups on the hEPC surface via copper-free click chemistry, resulting Cy5-hEPCs. The bioorthogonally labeled Cy5-hEPCs showed strong NIRF signal without cytotoxicity and functional perturbation in tubular formation, oxygen consumption and paracrine effect of hEPCs in vitro. In hind limb ischemia models, the distribution and migration of transplanted Cy5-hEPCs were successfully monitored via fluorescence molecular tomography (FMT) for 28 days. Notably, blood reperfusion and therapeutic neovascularization effects were significantly correlated with the initial transplantation forms of Cy5-hEPCs such as ‘condensed round shape’ and ‘spread shape’ in the ischemic lesion. The condensed transplanted Cy5-hEPCs substantially increased the therapeutic efficacy of hind limb ischemia, compared to that of spread Cy5-hEPCs. Therefore, our new stem cell labeling strategy can be used to predict therapeutic efficacy in hind limb ischemia and it can be applied a potential application in developing cell therapeutics for regenerative medicine.



논문확인(클릭시 이동) 
전체 52
번호 제목 작성자 작성일 추천 조회
52
[특허] 콘케이브와 진탕배양 기법으로 형성된 전분화능 줄기세포 유래 신경세포 제조방법 및 이에 의해 제조된 3D 신경 오가노이드를 통한 약물 독성 평가방법
관리자 | 2024.02.22 | 추천 0 | 조회 40
관리자 2024.02.22 0 40
51
[특허] 중간엽줄기세포 패치 공배양 기법을 이용한 투명 배상체 유래 간세포 제조방법 및 이에 의해 제조된 3D 간 오가노이드를 통한 약물 독성 평가방법
관리자 | 2024.02.22 | 추천 0 | 조회 39
관리자 2024.02.22 0 39
50
[특허] 인간 전분화능 줄기세포로 유래된 심장 오가노이드 제조방법, 효능 및 독성 평가 기술
관리자 | 2024.02.22 | 추천 0 | 조회 38
관리자 2024.02.22 0 38
49
[특허] 인공 신경망을 이용한 심장 부정맥 유발약물의 스크리닝 방법
관리자 | 2024.02.20 | 추천 0 | 조회 44
관리자 2024.02.20 0 44
48
[특허] 3 차원 세포 배양용 미세유체칩 및 이를 이용한 3 차원 세포 배양방법
관리자 | 2024.02.20 | 추천 0 | 조회 44
관리자 2024.02.20 0 44
47
Translation reinitiating in c.453delC frameshift mutation of KCNH2 producing functional hERG K+ channels with mild dominant neg
관리자 | 2024.02.02 | 추천 0 | 조회 49
관리자 2024.02.02 0 49
46
Extracellular matrix cues regulate the differentiation of pluripotent stem cell-derived endothelial cells.
관리자 | 2024.02.02 | 추천 0 | 조회 52
관리자 2024.02.02 0 52
45
Ultraefficient extracellular vesicle–guided direct reprogramming of fibroblasts into functional cardiomyocytes.
관리자 | 2024.02.02 | 추천 0 | 조회 52
관리자 2024.02.02 0 52
44
In vitro maturation of human pluripotent stem cell-derived cardiomyocyte: A promising approach for cell therapy.
관리자 | 2024.02.02 | 추천 0 | 조회 63
관리자 2024.02.02 0 63
43
Enhancement strategy for effective vascular regeneration following myocardial infarction through a dual stem cell approach.
관리자 | 2024.02.02 | 추천 0 | 조회 59
관리자 2024.02.02 0 59