특허 및 논문

바이오솔빅스에 자료실을 확인해 보세요.
bt_bb_section_bottom_section_coverage_image

BIOSOLVIX ARCHIVE특허 및 논문

Therapeutic Efficacy of Human Embryonic Stem Cell-Derived Endothelial Cells in Humanized Mouse Models Harboring a Human Immune

작성자
관리자
작성일
2024-01-31 16:50
조회
212
Yang HM, Moon SH, Choi YS, Park SJ, Lee YS, Lee HJ, Kim SJ, Chung HM. Therapeutic Efficacy of Human Embryonic Stem Cell-Derived Endothelial Cells in Humanized Mouse Models Harboring a Human Immune System. Arterioscl Throm Vas. 2013 Dec33(12):2839-49.

Abstract



Objective—

Allogeneic transplantation of human embryonic stem cell (hESC) derivatives has the potential to elicit the patient’s immune response and lead to graft rejection. Although hESCs and their derivatives have been shown to have advantageous immune properties in vitro, such observations could not be determined experimentally in vivo because of ethical and technical constraints. However, the generation of humanized mice (hu-mice) harboring a human immune system has provided a tool to perform in vivo immunologic studies of human cells and tissues. Using this model, we sought to examine the therapeutic potential of hESC-derived endothelial cells, human embryonic fibroblasts, and cord blood–derived endothelial progenitor cells in a human immune system environment.

Approach and Results—

All cell types transplanted in hu-mice showed significantly reduced cell survival during the first 14 days post-transplantation compared with that observed in immunodeficient mice. During this period, no observable therapeutic effects were detected in the hindlimb ischemic mouse models. After this point, the cells demonstrated improved survival and contributed to a long-term improvement in blood perfusion. All cell types showed reduced therapeutic efficacy in hu-mice compared with NOD scid IL2 receptor gamma chain knockout mice. Interestingly, the eventual improvement in blood flow caused by the hESC-derived endothelial cells in hu-mice was not much lower than that observed in NOD scid IL2 receptor gamma chain knockout mice.

Conclusions—

These findings suggest that hESC derivatives may be considered a good source for cell therapy and that hu-mice could be used as a preclinical in vivo animal model for the evaluation of therapeutic efficacy to predict the outcomes of human clinical trials.


Keywords

#endothelial cells #human embryonic stem cells #hindlimb ischemia #humanized mouse #therapeutic efficacy

 

논문확인(클릭시 이동)
전체 52
번호 제목 작성자 작성일 추천 조회
12
Therapeutic Efficacy of Human Embryonic Stem Cell-Derived Endothelial Cells in Humanized Mouse Models Harboring a Human Immune
관리자 | 2024.01.31 | 추천 0 | 조회 212
관리자 2024.01.31 0 212
11
Development of a xeno- free autologous culture system for endothelial progenitor cells derived from human umbilical cord blood.
관리자 | 2024.01.31 | 추천 0 | 조회 234
관리자 2024.01.31 0 234
10
The use of aggregates of purified cardiomyocytes derived from human ESCs for functional engraftment after myocardial infarction
관리자 | 2024.01.31 | 추천 0 | 조회 186
관리자 2024.01.31 0 186
9
Pertussis Toxin Enhances Colony Organization of Enzymatic-dissociated Single Human Embryonic Stem Cells.
관리자 | 2024.01.31 | 추천 0 | 조회 204
관리자 2024.01.31 0 204
8
Effective Method for the Isolation and Proliferation of Primary Lung Cancer Cells from Patient Lung Tissues.
관리자 | 2024.01.31 | 추천 0 | 조회 167
관리자 2024.01.31 0 167
7
Modification of a purification and expansion method for human embryonic stem cell-derived cardiomyocytes.
관리자 | 2024.01.31 | 추천 0 | 조회 183
관리자 2024.01.31 0 183
6
A comparison of human cord blood- and embryonic stem cell-derived endothelial progenitor cells in the treatment of chronic woun
관리자 | 2024.01.31 | 추천 0 | 조회 185
관리자 2024.01.31 0 185
5
Effect of chromosome instability on the maintenance and differentiation of human embryonic stem cells in vitro and in vivo.
관리자 | 2024.01.31 | 추천 0 | 조회 230
관리자 2024.01.31 0 230
4
A system for treating ischemic disease using human embryonic stem cell-derived endothelial cells without direct incorporation.
관리자 | 2024.01.31 | 추천 0 | 조회 260
관리자 2024.01.31 0 260
3
Gene Expression Profiles in CHA3 and CHA4 Human Embryonic Stem Cells and Embryoid Bodies
관리자 | 2024.01.31 | 추천 0 | 조회 218
관리자 2024.01.31 0 218