특허 및 논문

바이오솔빅스에 자료실을 확인해 보세요.
bt_bb_section_bottom_section_coverage_image

BIOSOLVIX ARCHIVE특허 및 논문

Modification of a purification and expansion method for human embryonic stem cell-derived cardiomyocytes.

작성자
관리자
작성일
2024-01-31 15:36
조회
185
Park SJ*, Bae DK*, Moon SH, Chung HM*. Modification of a purification and expansion method for human embryonic stem cell-derived cardiomyocytes. Cardiology. 2013124(3):139-50.

 

Objective: This study aimed to develop a simple and efficient purification method for human embryonic stem cell (hESC)-derived cardiomyocytes (CMs) using a low-glucose culture system. In addition, we investigated whether intercellular adhesion between single hESC-CMs plays a critical role in enhancing proliferation of purified hESC-CMs. Method: hESCs were cultured in suspension to form human embryoid bodies (hEBs) from which ∼15% contracting clusters were derived after 15-20 days in culture. To purify CMs from contracting hEBs, we first manually isolated contracting clumps that were re-cultured on gelatin-coated plates with media containing a low concentration of glucose. The purified hESC-CMs were cultured at different densities to examine whether cell-cell contact enhances proliferation of hESC-CMs. Results: Purified CMs demonstrated spontaneous contraction and strongly expressed the CM-specific markers cardiac troponin T and slow myosin heavy chain. We investigated the purification efficiency by examining the expression levels of cardiac-related genes and the expression of MitoTracker Red dye. In addition, purified hESC-CMs in low-glucose culture demonstrated a 1.5-fold increase in their proliferative capacity compared to those cultured as single hESC-CMs. Conclusion: A low level of glucose is efficient in purifying hESC-CMs and intercellular adhesion between individual hESC-CMs plays a critical role in enhancing hESC-CM proliferation.



#Cardiomyocytes, #Expansion, #Human embryonic stem cells, #Purification



 

논문확인 (클릭시 이동)
전체 52
번호 제목 작성자 작성일 추천 조회
12
Therapeutic Efficacy of Human Embryonic Stem Cell-Derived Endothelial Cells in Humanized Mouse Models Harboring a Human Immune
관리자 | 2024.01.31 | 추천 0 | 조회 213
관리자 2024.01.31 0 213
11
Development of a xeno- free autologous culture system for endothelial progenitor cells derived from human umbilical cord blood.
관리자 | 2024.01.31 | 추천 0 | 조회 236
관리자 2024.01.31 0 236
10
The use of aggregates of purified cardiomyocytes derived from human ESCs for functional engraftment after myocardial infarction
관리자 | 2024.01.31 | 추천 0 | 조회 187
관리자 2024.01.31 0 187
9
Pertussis Toxin Enhances Colony Organization of Enzymatic-dissociated Single Human Embryonic Stem Cells.
관리자 | 2024.01.31 | 추천 0 | 조회 205
관리자 2024.01.31 0 205
8
Effective Method for the Isolation and Proliferation of Primary Lung Cancer Cells from Patient Lung Tissues.
관리자 | 2024.01.31 | 추천 0 | 조회 169
관리자 2024.01.31 0 169
7
Modification of a purification and expansion method for human embryonic stem cell-derived cardiomyocytes.
관리자 | 2024.01.31 | 추천 0 | 조회 185
관리자 2024.01.31 0 185
6
A comparison of human cord blood- and embryonic stem cell-derived endothelial progenitor cells in the treatment of chronic woun
관리자 | 2024.01.31 | 추천 0 | 조회 186
관리자 2024.01.31 0 186
5
Effect of chromosome instability on the maintenance and differentiation of human embryonic stem cells in vitro and in vivo.
관리자 | 2024.01.31 | 추천 0 | 조회 232
관리자 2024.01.31 0 232
4
A system for treating ischemic disease using human embryonic stem cell-derived endothelial cells without direct incorporation.
관리자 | 2024.01.31 | 추천 0 | 조회 262
관리자 2024.01.31 0 262
3
Gene Expression Profiles in CHA3 and CHA4 Human Embryonic Stem Cells and Embryoid Bodies
관리자 | 2024.01.31 | 추천 0 | 조회 219
관리자 2024.01.31 0 219