특허 및 논문

바이오솔빅스에 자료실을 확인해 보세요.
bt_bb_section_bottom_section_coverage_image

BIOSOLVIX ARCHIVE특허 및 논문

A system for treating ischemic disease using human embryonic stem cell-derived endothelial cells without direct incorporation.

작성자
관리자
작성일
2024-01-31 14:44
조회
261
Moon SH*, Kim JS*, Park SJ, Lee HJ, Do JT, Chung HM. A system for treating ischemic disease using human embryonic stem cell-derived endothelial cells without direct incorporation. Biomaterials. 2011 32:6445-6455.

Abstract

Despite studies on the use of human embryonic stem cell (hESC) derivatives to treat ischemic diseases, there are technical safety issues that have yet to be resolved. Herein, we sought to develop a method for using secreted angiogenic factors from hESC-derived endothelial cell derivatives (hESC-ECs), while avoiding direct cell incorporation, to reduce tumorigenesis or unidentified side effects of injected cells in vivo. Multicellular aggregation of hESC-ECs (MA-hESC-ECs) increases survivability, and encapsulation in Matrigel (EnMA-hESC-ECs) blocks the cells’ in vivo migration. To examine the therapeutic effects of EnMA-hESC-ECs, we implanted both forms of hESC-ECs in a mouse model of hindlimb ischemia. Treatment with EnMA-hESC-ECs suppressed limb loss and tissue damage with no noticeable side effects, such as tumorigenesis or teratoma formation, and the Matrigel implant could be easily removed after the procedure. Thus, MA and the encapsulation system are effective techniques for utilizing humoral factors secreted by hESC derivatives that aid in the survivability and safety. We expect these results to contribute to the thriving stem cell field by improving the bioavailability of hESC derivatives for regenerative medicine.



논문확인 (클릭시 이동)
전체 52
번호 제목 작성자 작성일 추천 조회
12
Therapeutic Efficacy of Human Embryonic Stem Cell-Derived Endothelial Cells in Humanized Mouse Models Harboring a Human Immune
관리자 | 2024.01.31 | 추천 0 | 조회 212
관리자 2024.01.31 0 212
11
Development of a xeno- free autologous culture system for endothelial progenitor cells derived from human umbilical cord blood.
관리자 | 2024.01.31 | 추천 0 | 조회 234
관리자 2024.01.31 0 234
10
The use of aggregates of purified cardiomyocytes derived from human ESCs for functional engraftment after myocardial infarction
관리자 | 2024.01.31 | 추천 0 | 조회 186
관리자 2024.01.31 0 186
9
Pertussis Toxin Enhances Colony Organization of Enzymatic-dissociated Single Human Embryonic Stem Cells.
관리자 | 2024.01.31 | 추천 0 | 조회 204
관리자 2024.01.31 0 204
8
Effective Method for the Isolation and Proliferation of Primary Lung Cancer Cells from Patient Lung Tissues.
관리자 | 2024.01.31 | 추천 0 | 조회 168
관리자 2024.01.31 0 168
7
Modification of a purification and expansion method for human embryonic stem cell-derived cardiomyocytes.
관리자 | 2024.01.31 | 추천 0 | 조회 183
관리자 2024.01.31 0 183
6
A comparison of human cord blood- and embryonic stem cell-derived endothelial progenitor cells in the treatment of chronic woun
관리자 | 2024.01.31 | 추천 0 | 조회 185
관리자 2024.01.31 0 185
5
Effect of chromosome instability on the maintenance and differentiation of human embryonic stem cells in vitro and in vivo.
관리자 | 2024.01.31 | 추천 0 | 조회 231
관리자 2024.01.31 0 231
4
A system for treating ischemic disease using human embryonic stem cell-derived endothelial cells without direct incorporation.
관리자 | 2024.01.31 | 추천 0 | 조회 261
관리자 2024.01.31 0 261
3
Gene Expression Profiles in CHA3 and CHA4 Human Embryonic Stem Cells and Embryoid Bodies
관리자 | 2024.01.31 | 추천 0 | 조회 218
관리자 2024.01.31 0 218