특허 및 논문

바이오솔빅스에 자료실을 확인해 보세요.
bt_bb_section_bottom_section_coverage_image

BIOSOLVIX ARCHIVE특허 및 논문

Non-invasive stem cell tracking in hindlimb ischemia animal model using bio- orthogonal copper-free click chemistry.

작성자
관리자
작성일
2024-02-01 10:10
조회
236
Lee SY, Lee S, Lee J, Yhee JY, Yoon HI, Park SJ, Koo H, Moon SH, Lee H, Cho YW, Kang SW, Lee SY, Kim K*. Non-invasive stem cell tracking in hindlimb ischemia animal model using bio- orthogonal copper-free click chemistry. Biochem Biophys Res Commun. 2016 Oct 28;479(4):779-786.

Abstract


Labeling of stem cells aims to distinguish transplanted cells from host cells, understand in vivo fate of transplanted cells, particularly important in stem cell therapy. Adipose-derived mesenchymal stem cells (ASCs) are considered as an emerging therapeutic option for tissue regeneration, but much remains to be understood regarding the in vivo evidence. In this study, a simple and efficient cell labeling method for labeling and tracking of stem cells was developed based on bio-orthogonal copper-free click chemistry, and it was applied in a mouse hindlimb ischemia model. The human ASCs were treated with tetra-acetylated N-azidoacetyl-d-mannosamine (Ac4ManNAz) to generate glycoprotein with unnatural azide groups on the cell surface, and the generated azide groups were fluorescently labeled by specific binding of dibenzylcyclooctyne-conjugated Cy5 (DBCO-Cy5). The safe and long-term labeling of the hASCs by this method was first investigated in vitro. Then the DBCO-Cy5-hASCs were transplanted into the hindlimb ischemia mice model, and we could monitor and track in vivo fate of the cells using optical imaging system. We could clearly observe the migration potent of the hASCs toward the ischemic lesion. This approach to design and tailor new method for labeling of stem cells may be useful to provide better understanding on the therapeutic effects of transplanted stem cells into the target diseases.



Keywords: #Bio-orthogonal copper-free click chemistry #Cell labeling and tracking #Hindlimb ischemia #Mesenchymal stem cell #Metabolic glycoengineering.

논문확인(클릭시 이동)
전체 52
번호 제목 작성자 작성일 추천 조회
22
Photodynamic Approach for Teratoma-Free Pluripotent Stem Cell Therapy Using CDy1 and Visible Light.
관리자 | 2024.02.01 | 추천 0 | 조회 235
관리자 2024.02.01 0 235
21
Non-invasive stem cell tracking in hindlimb ischemia animal model using bio- orthogonal copper-free click chemistry.
관리자 | 2024.02.01 | 추천 0 | 조회 236
관리자 2024.02.01 0 236
20
Spontaneous inward currents reflecting oscillatory activation of Na/Ca2 exchangers in human embryonic stem cell-derived cardiom
관리자 | 2024.02.01 | 추천 0 | 조회 280
관리자 2024.02.01 0 280
19
Examination of endothelial cell-induced epidermal regeneration in a mice-based chimney wound model.
관리자 | 2024.02.01 | 추천 0 | 조회 213
관리자 2024.02.01 0 213
18
Repair of Ischemic Injury by Pluripotent Stem Cell Based Cell Therapy without Teratoma through selective Photosensitivity.
관리자 | 2024.02.01 | 추천 0 | 조회 248
관리자 2024.02.01 0 248
17
Well-defined differentiation of hESC-derived hemangioblasts by embryoid body formation without enzymatic treatment.
관리자 | 2024.02.01 | 추천 0 | 조회 194
관리자 2024.02.01 0 194
16
Role of bone marrow-derived progenitor cells in de novo liver regeneration in human liver transplants.
관리자 | 2024.02.01 | 추천 0 | 조회 233
관리자 2024.02.01 0 233
15
Optimizing human embryonicstem cells differentiation efficiency by screening size-tunable homogenous embryoid bodies.
관리자 | 2024.01.31 | 추천 0 | 조회 250
관리자 2024.01.31 0 250
14
Microchip-based engineering of super - pancreatic islets supported by adipose-derived stem cells.
관리자 | 2024.01.31 | 추천 0 | 조회 247
관리자 2024.01.31 0 247
13
Nanotopographical control for maintaining undifferentiated human embryonic stem cell colonies in feeder free conditions.
관리자 | 2024.01.31 | 추천 0 | 조회 242
관리자 2024.01.31 0 242