특허 및 논문

바이오솔빅스에 자료실을 확인해 보세요.
bt_bb_section_bottom_section_coverage_image

BIOSOLVIX ARCHIVE특허 및 논문

Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivat

작성자
관리자
작성일
2024-02-01 21:43
조회
288

. Jo HY, Han HW, Jung I, Ju JH, Park SJ, Moon SH, Geum D, Kim H, Park HJ, Kim S, Stacey GN, Koo SK, Park MH, Kim JH. Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivatives. Sci Rep. 2020 Mar 3;10(1):3939.


Abstract



Although human induced pluripotent stem cell (hiPSC) lines are karyotypically normal, they retain the potential for mutation in the genome. Accordingly, intensive and relevant quality controls for clinical-grade hiPSCs remain imperative. As a conceptual approach, we performed RNA-seq-based broad-range genetic quality tests on GMP-compliant human leucocyte antigen (HLA)-homozygous hiPSCs and their derivatives under postdistribution conditions to investigate whether sequencing data could provide a basis for future quality control. We found differences in the degree of single-nucleotide polymorphism (SNP) occurring in cells cultured at three collaborating institutes. However, the cells cultured at each centre showed similar trends, in which more SNPs occurred in late-passage hiPSCs than in early-passage hiPSCs after differentiation. In eSNP karyotyping analysis, none of the predicted copy number variations (CNVs) were identified, which confirmed the results of SNP chip-based CNV analysis. HLA genotyping analysis revealed that each cell line was homozygous for HLA-A, HLA-B, and DRB1 and heterozygous for HLA-DPB type. Gene expression profiling showed a similar differentiation ability of early- and late-passage hiPSCs into cardiomyocyte-like, hepatic-like, and neuronal cell types. However, time-course analysis identified five clusters showing different patterns of gene expression, which were mainly related to the immune response. In conclusion, RNA-seq analysis appears to offer an informative genetic quality testing approach for such cell types and allows the early screening of candidate hiPSC seed stocks for clinical use by facilitating safety and potential risk evaluation.


논문확인(클릭시 이동)
전체 52
번호 제목 작성자 작성일 추천 조회
42
Multiple isogenic GNE-myopathy modeling with mutation specific phenotypes from human pluripotent stem cells by base editors.
관리자 | 2024.02.02 | 추천 0 | 조회 232
관리자 2024.02.02 0 232
41
In silico design and fabrication of an SFI chip-based microspheroid culture system.
관리자 | 2024.02.02 | 추천 0 | 조회 289
관리자 2024.02.02 0 289
40
Effect and application of cryopreserved three-dimensional microcardiac spheroids in myocardial infarction therapy
관리자 | 2024.02.01 | 추천 0 | 조회 231
관리자 2024.02.01 0 231
39
Blue laser-induced selective vasorelaxation by the activation of NOSs.
관리자 | 2024.02.01 | 추천 0 | 조회 241
관리자 2024.02.01 0 241
38
Therapeutic Efficacy of Artificial Skin Produced by 3D Bioprinting.
관리자 | 2024.02.01 | 추천 0 | 조회 267
관리자 2024.02.01 0 267
37
Cardiovascular events and safety outcomes associated with remdesivir using a World Health Organization  international pharmacov
관리자 | 2024.02.01 | 추천 0 | 조회 237
관리자 2024.02.01 0 237
36
Predicting in vivo therapeutic efficacy of bioorthogonally labeled endothelial progenitor cells in hind limb ischemia models vi
관리자 | 2024.02.01 | 추천 0 | 조회 228
관리자 2024.02.01 0 228
35
Application of co-culture technology of epithelial type cells and mesenchymal type cells using nanopatterned structures
관리자 | 2024.02.01 | 추천 0 | 조회 264
관리자 2024.02.01 0 264
34
Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivat
관리자 | 2024.02.01 | 추천 0 | 조회 288
관리자 2024.02.01 0 288
33
Antiviral activity and safety of remdesivir against SARS-CoV- 2 infection in human pluripotent stem cell-derived cardiomyocytes
관리자 | 2024.02.01 | 추천 0 | 조회 248
관리자 2024.02.01 0 248