특허 및 논문

바이오솔빅스에 자료실을 확인해 보세요.
bt_bb_section_bottom_section_coverage_image

BIOSOLVIX ARCHIVE특허 및 논문

Enhancement strategy for effective vascular regeneration following myocardial infarction through a dual stem cell approach.

작성자
관리자
작성일
2024-02-02 08:29
조회
316
Hyeok Kim*, Soon-Jung Park*, Jae-Hyun Park, Sunghun Lee, Bong-Woo Park, Soon Min Lee, Ji-Won Hwang, Jin-Ju Kim, Byeongmin Kang, Woo-Sup Sim, Hyo-Jin Kim, Seung Hwan Jeon, Dong-Bin Kim, Jinah Jang, Dong-Woo Cho, Sung-Hwan Moon*, Hun-Jun Park*, Kiwon Ban*. Enhancement strategy for effective vascular regeneration following myocardial infarction through a dual stem cell approach. Experimental & Molecular Medicine volume 54, pages1165–1178 (2022).

 

Abstract



Since an impaired coronary blood supply following myocardial infarction (MI) negatively affects heart function, therapeutic neovascularization is considered one of the major therapeutic strategies for cell-based cardiac repair. Here, to more effectively achieve therapeutic neovascularization in ischemic hearts, we developed a dual stem cell approach for effective vascular regeneration by utilizing two distinct types of stem cells, CD31+-endothelial cells derived from human induced pluripotent stem cells (hiPSC-ECs) and engineered human mesenchymal stem cells that continuously secrete stromal derived factor-1α (SDF-eMSCs), to simultaneously promote natal vasculogenesis and angiogenesis, two core mechanisms of neovascularization. To induce more comprehensive vascular regeneration, we intramyocardially injected hiPSC-ECs to produce de novo vessels, possibly via vasculogenesis, and a 3D cardiac patch encapsulating SDF-eMSCs (SDF-eMSC-PA) to enhance angiogenesis through prolonged secretion of paracrine factors, including SDF-1α, was implanted into the epicardium of ischemic hearts. We verified that hiPSC-ECs directly contribute to de novo vessel formation in ischemic hearts, resulting in enhanced cardiac function. In addition, the concomitant implantation of SDF1α-eMSC-PAs substantially improved the survival, retention, and vasculogenic potential of hiPSC-ECs, ultimately achieving more comprehensive neovascularization in the MI hearts. Of note, the newly formed vessels through the dual stem cell approach were significantly larger and more functional than those formed by hiPSC-ECs alone. In conclusion, these results provide compelling evidence that our strategy for effective vascular regeneration can be an effective means to treat ischemic heart disease.


 

논문확인(클릭시 이동) 

 
전체 52
번호 제목 작성자 작성일 추천 조회
52
[특허] 콘케이브와 진탕배양 기법으로 형성된 전분화능 줄기세포 유래 신경세포 제조방법 및 이에 의해 제조된 3D 신경 오가노이드를 통한 약물 독성 평가방법
관리자 | 2024.02.22 | 추천 0 | 조회 257
관리자 2024.02.22 0 257
51
[특허] 중간엽줄기세포 패치 공배양 기법을 이용한 투명 배상체 유래 간세포 제조방법 및 이에 의해 제조된 3D 간 오가노이드를 통한 약물 독성 평가방법
관리자 | 2024.02.22 | 추천 0 | 조회 279
관리자 2024.02.22 0 279
50
[특허] 인간 전분화능 줄기세포로 유래된 심장 오가노이드 제조방법, 효능 및 독성 평가 기술
관리자 | 2024.02.22 | 추천 0 | 조회 310
관리자 2024.02.22 0 310
49
[특허] 인공 신경망을 이용한 심장 부정맥 유발약물의 스크리닝 방법
관리자 | 2024.02.20 | 추천 0 | 조회 317
관리자 2024.02.20 0 317
48
[특허] 3 차원 세포 배양용 미세유체칩 및 이를 이용한 3 차원 세포 배양방법
관리자 | 2024.02.20 | 추천 0 | 조회 286
관리자 2024.02.20 0 286
47
Translation reinitiating in c.453delC frameshift mutation of KCNH2 producing functional hERG K+ channels with mild dominant neg
관리자 | 2024.02.02 | 추천 0 | 조회 285
관리자 2024.02.02 0 285
46
Extracellular matrix cues regulate the differentiation of pluripotent stem cell-derived endothelial cells.
관리자 | 2024.02.02 | 추천 0 | 조회 339
관리자 2024.02.02 0 339
45
Ultraefficient extracellular vesicle–guided direct reprogramming of fibroblasts into functional cardiomyocytes.
관리자 | 2024.02.02 | 추천 0 | 조회 358
관리자 2024.02.02 0 358
44
In vitro maturation of human pluripotent stem cell-derived cardiomyocyte: A promising approach for cell therapy.
관리자 | 2024.02.02 | 추천 0 | 조회 372
관리자 2024.02.02 0 372
43
Enhancement strategy for effective vascular regeneration following myocardial infarction through a dual stem cell approach.
관리자 | 2024.02.02 | 추천 0 | 조회 316
관리자 2024.02.02 0 316